【文獻標(biāo)題】Isolation and identification of senescent renal tubular epithelial cells using immunomagnetic beads based on DcR2

【作者】Jia Chen，Ke-Hong Chen，Bi-Qiong Fu，et.al

【作者單位】第三軍醫(yī)大學(xué)（Third Military Medical University）

【文獻中引用產(chǎn)品】

人白細胞介素6（IL-6）ELISA試劑盒

人轉(zhuǎn)化生長因子β1（TGF-β1）ELISA試劑盒

【關(guān)鍵詞】Cell senescence，Renal tubular epithelial cell，Cell sorting，Decoy receptor 2

【DOI】dx.doi.org/10.1016/j.exger.2017.04.008

【影響因子(IF)】5.10

【出版期刊】《Experimental Gerontology》

【產(chǎn)品原文引用】

Cell supernatants were collected from the control group, passage 5,AGEs and H2O2 groups. After the adherence of MACS-sorted DcR2-positive and negative cells, normal medium was added. The cell supernatant of the two groups was collected after 48 h. The levels of IL-6 and TGF-β1 in the supernatant were measured by ELISA kit （Hengyuan,Shanghai, China）, according to the manufacturer's instructions.

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